Identifiler Direct
Validated Protocol for Processing with Identifiler® Direct
The following protocol has been validated for processing the Bode Buccal DNA Collector with the AmpFlSTR® Identifiler® Direct PCR Amplification Kit.
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Remove one 1.2mm punch from the Buccal DNA Collector and place into tube or PCR plate well. |
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Add Bode PunchPrep Solution (Cat No. P09D79) and incubate on a 70oC heat block for 20 minutes. |
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Add 25µl of AmpFlSTR® Identifiler® Direct reaction mix to the 1.2 mm punch and perform a *26-cycle PCR reaction according to the instructions found in the AmpFlSTR® Identifiler® Direct PCR Amplifcation Kit User Guide. |
*It is recommended that internal validation and testing regarding the number of PCR cycles is performed by each agency. The number of cycles within this protocol should serve as a recommendation for processing.
Validation Results
| Bode Buccal DNA Collector | FTA Sample | |
| Number of Samples | 88 | 88 |
| 1st Pass Success Rate | 84% | 82% |
The Buccal DNA Collector provided equivalent performance to FTA when processing paired samples using the AmpFlSTR® Identifiler® Direct PCR Amplification Kit.
Electropherogram 1: Sample from the Buccal DNA Collector – processed with AmpFlSTR® Identifiler® Direct PCR Amplification Kit
Electropherogram 2: Sample from the same donor collected on FTA paper – processed with AmpFlSTR® Identifiler® Direct PCR
AmpFlSTR® and Identifiler® are registered trade marks of Life Technologies Corporation
