Validated Protocol for Processing with AmpFlSTR®  Identifiler® Direct

The following protocol has been validated for processing the Bode Buccal DNA Collector with the AmpFlSTR® Identifiler® Direct PCR Amplification Kit.

	Add 2µl of Applied Biosystem’s Prep-N-Go™ Buffer into tube or PCR plate well.
	Remove one 1.2mm punch from the Buccal DNA Collector and place into tube or PCR plate well containing the Prep-N-Go™ Buffer.  Ensure that the punch i s immersed in the buffer on the bottom of the tube or PCR plate well.  If it is not, cover tube or PCR plate and briefly centrifuge.
	Add 25µl of AmpFlSTR® Identifiler® Direct reaction mix to the tube or PCR plate well and perform a *26-cycle PCR reaction and prepare sample(s) for electrophoresis according to the instructions found in the AmpFlSTR® Identifiler® Direct PCR Amplifcation Kit User Guide. *It is recommended that internal validation and testing regarding the number of PCR cycles is performed by each agency. The number of cycles within this protocol should serve as a recommendation for processing.
	Analyze the sample(s) according to the approved software and analysis guidelines of the agency.

Validation Results

Results from Prep-N-Go™ Buffer/AmpFlSTR® Identifiler® Direct evaluation.  159 samples were processed following the Prep-N-Go™/ Identifiler® Direct procedure.

AmpFlSTR® and Identifiler® are registered trademarks of Life Technologies Corporation

DNA Profile of a sample processed with Prep-N-Go™ Buffer/AmpFlSTR® Identifiler® Direct

Accreditations