SecurSwab™ DUO-V Swab System
Processing with AmpFlSTR® Identifiler® Direct
Validated Protocol for Processing with AmpFlSTR® Identifiler® Direct using Prep-N-Go®
The following protocol has been validated for processing the Bode Buccal DNA Collector with the AmpFlSTR® Identifiler® Direct PCR Amplification Kit.
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Add 2µl of Applied Biosystem’s Prep-N-Go™ Buffer into tube or PCR plate well. |
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Remove one 1.2mm punch from the Buccal DNA Collector and place into tube or PCR plate well containing the Prep-N-Go™ Buffer. Ensure that the punch i s immersed in the buffer on the bottom of the tube or PCR plate well. If it is not, cover tube or PCR plate and briefly centrifuge. |
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Add 25µl of AmpFlSTR® Identifiler® Direct reaction mix to the tube or PCR plate well and perform a *26-cycle PCR reaction and prepare sample(s) for electrophoresis according to the instructions found in the AmpFlSTR® Identifiler® Direct PCR Amplifcation Kit User Guide. *It is recommended that internal validation and testing regarding the number of PCR cycles is performed by each agency. The number of cycles within this protocol should serve as a recommendation for processing. |
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Analyze the sample(s) according to the approved software and analysis guidelines of the agency. |
Validated Protocol for Processing with AmpFlSTR® Identifiler® Direct using Bode PunchPrep
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Remove one 1.2mm punch from the Buccal DNA Collector and place into tube or PCR plate well. |
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Add Bode PunchPrep Solution (Cat No. P09D79) and incubate on a 70oC heat block for 20 minutes. |
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Add 25µl of AmpFlSTR Identifiler reaction mix to the 1.2 mm punch and perform a *26-cycle PCR reaction according to the instructions found in the AmpFlSTR® Identifiler® Direct PCR Amplifcation Kit User Guide. *It is recommended that internal validation and testing regarding the number of PCR cycles is performed by each agency. The number of cycles within this protocol should serve as a recommendation for processing. |
Validation Results
Results from Prep-N-Go™ Buffer/AmpFlSTR® Identifiler® Direct evaluation. 159 samples were processed following the Prep-N-Go™/ Identifiler® Direct procedure.
| Number of Amplification Failures | Number of Homozygote Off-Scale Profiles | 1st Pass Success Rate | ||
| Mixture Profile | Partial Profile | No Profile | Heterozygotes: >75 RFU Homozygotes: > 200 RFU | |
| 0 | 0 | 0 | 29 | 100% |
AmpFlSTR® and Identifiler® are registered trademarks of Life Technologies Corporation
DNA Profile of a sample processed with Prep-N-Go™ Buffer/AmpFlSTR® Identifiler® Direct
Accreditations
