Bode PunchPrep™

Bode Punch Prep – For Direct-to-Amplification Processing Using Bode Buccal DNA Collectors

Bode PunchPrep enhances the use of the Bode Buccal DNA Collector with direct amplification chemistries. Bode’s validated protocols for processing the Buccal DNA collector with the AmpFlSTR® Identifiler® Direct PCR Amplification kit from Life Technologies and the PowerPlex®16HS & PowerPlex®18D Systems from Promega provide first pass success rates of greater than 80% – equivalent to the success rate processing samples on FTA paper.

Click Here for the Bode PunchPrep User Manual

Processing with Bode PunchPrep

Bode PunchPrep has been developed so that Bode Buccal DNA Collectors can be processed with Direct-to-Amplification chemistries, without significant changes to the protocol. Bode PunchPrep has been validated with Manual and Automated processes. The overall protocol for processing is simple:

Remove a 1.2mm punch from the Bode Buccal DNA Collector.
Apply 2uL of Bode PunchPrep directly to the punch.
Heat at 70C to dry the punch.
Begin the PCR reaction as described in the Identifiler Direct/ PowerPlex 16 HS User Manuals.

Validated Protocols

Click Here for Details on processing with Identifiler Direct
Click Here for Details on processing with PowerPlex 16 HS

Accuracy

Bode analyzed the samples for accuracy of profiles as a part of the validation. The accuracy of the samples was 100%.

Sensitivity

Bode added known amounts of cells to the collector and processed using Bode PunchPrep and a direct-to-amplification chemistry. Bode used known cell counts of 50 cells, 100 cells, 200 cells, 400 cells, 800 cells, and 1600 cells.

Reproducibility

Bode processed 8 samples from the same collector to ensure the peak heights and balances are similar in each trial. Variability is anticipated due to natural gradients that occur during collection – as each sample is punched from a different area of the collector.

Ordering Information

Product	Part Number	Quantity
Bode PunchPrep	P09D79	12 X 0.5mL – One Time Use Bode PunchPrep Vials Each vial is sufficient for 100 reactions

Contact Bode for additional information.