Bode PunchPrep enhances the use of the Bode Buccal DNA Collector with direct amplification chemistries. Bode’s validated protocols for processing the Buccal DNA collector with the AmpFlSTR® Identifiler® Direct PCR Amplification kit from Life Technologies and the PowerPlex®16HS & PowerPlex®18D Systems from Promega provide first pass success rates of greater than 80% – equivalent to the success rate processing samples on FTA paper.
- Click Here for Processing with Identifiler® Direct
- Click Here for Processing with PowerPlex® 16HS, 18D, and 21
- Click Here for Flyer – Processing Bode Buccal DNA Collectors with Direct-to-Amplification Chemistries
Processing with Bode PunchPrep
|Bode PunchPrep has been developed so that Bode Buccal DNA Collectors can be processed with Direct-to-Amplification chemistries, without significant changes to the protocol. Bode PunchPrep has been validated with Manual and Automated processes. The overall protocol for processing is simple:
Bode analyzed the samples for accuracy of profiles as a part of the validation. The accuracy of the samples was 100%.
Bode added known amounts of cells to the collector and processed using Bode PunchPrep and a direct-to-amplification chemistry. Bode used known cell counts of 50 cells, 100 cells, 200 cells, 400 cells, 800 cells, and 1600 cells.
Bode processed 8 samples from the same collector to ensure the peak heights and balances are similar in each trial. Variability is anticipated due to natural gradients that occur during collection – as each sample is punched from a different area of the collector.
|Bode PunchPrep||P09D79||12 X 0.5mL – One Time Use Bode PunchPrep Vials Each vial is sufficient for 100 reactions|
Contact Bode for additional information.